小鼠胰岛素（INS）酶联免疫试剂盒-齐一生物
for research use only
仅供研究用
货号：qy-m30038
小鼠胰岛素(ins)酶联免疫分析(elisa)
试剂盒使用说明书
本试剂仅供研究使用 目的：本试剂盒用于测定小鼠血清，细胞上清及相关液体样本中胰岛素(ins)的含量。
实验原理：
本试剂盒应用双抗体夹心法测定标本中小鼠胰岛素(ins)水平。用纯化的小鼠抗-胰岛素(ins)抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入胰岛素(ins),再与hrp 标记的胰岛素（ins）抗体结合，形成抗体-抗原-酶标抗体复合物，经过*洗涤后加底物 tmb 显色。tmb 在hrp 酶的催化下转化成蓝色，并在酸的作用下转化成zui终的黄色。颜色的深浅和样品中的胰岛素(ins)呈正相关。用酶标仪在 450nm波长下测定吸光度（od值），通过标准曲线计算样品中小鼠胰岛素(ins)浓度。
specimen requirements
n
assay procedure
1.dilute and add sample to standard: set 10 standard wells on the elisa plates coated, add standard 100μl to the first and the second well, then add standard dilution 50μl to the first and the second well, mix; take out 100μl form the first and the second well then add it to the third and the forth well separay. then add standard dilution 50μl to the third and the forth well ,mix ; then take out 50μl from the third and the forth well discard, add 50μl to the fifth and the sixth well ,then add standard dilution 50μl to the fifth and the sixth well, mix ; take out 50μl from the fifth and the sixth well and add to the seventh and the eighth well, then add standard dilution 50μl to the seventh and the eighth well ,mix ; take out 50μl from the seventh and the eighth well and add to the ninth and the tenth well, add standard dilution 50μl to the ninth and the tenth well, mix , take out 50μl from the ninth and the tenth well discard(add sample 50μl to each well after diluting ,(density: 12 mu/l,8mu/l ,4 mu/l,2 mu/l, 1 mu/l)
2.add sample ：set blank wells separay (blank comparison wells don’t add sample and hrp-conjugate reagent, other each step operation is same). testing sample well. add sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, and gently mix.
3.add enzyme：add hrp-conjugate reagent 50μl to each well, except blank well.
4.incubate: after closing plate with closure plate membrane ,incubate for 30 min at 37℃. 5.configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water and reserve.
6.washing：uncover closure plate membrane, discard liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.
7.color：add chromogen solution a 50ul and chromogen solution b to each well, evade thelight preservation for 10 min at 37℃
8.stop the reaction ：add stop solution50μl to each well, stop the reaction(the blue color change to yellow color).
9.assay：take blank well as zero , read absorbance at 450nm after adding stop solution and within 15min.
important notes
the kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, elisa plates coated if has not use up after opened, the plate should be stored in sealed bag.washing buffer will crystallization separation, it can be heated the water helps dissolve when dilute . washing does not affect the result.add sample with sampler each step, and proofread its accuracy frequently, avoids the experimental error. add sample within 5 min, if the number of sample is much , recommend to use volley .if the testing material content is excessively higher (the sample od is bigger than the first standard well ),please dilute sample (n-fold), please diluente and multiplied by the dilution factor.（×n×5）.closure plate membrane only limits the disposable use, to avoid cross-contamination.the substrate evade the light preservation.please according to use instruction strictly, the test result determination must take the microtiter plate reader as a standard.all samples, washing buffer and each kind of reject should according to infective material process.do not mix reagents with those from other lots.assay range
0.3 mu/l -15 mu/l
storage and validity
1．storage： 2-8℃.
2．validity：six months.
齐一生物科技（上海）有限公司主要经营进口原装elisa试剂盒，自产品牌qiybo试剂盒，并提供免疫分析相关试剂和检测试剂盒。种类齐全，价格实惠，凡购买我公司产品的新老客户，即可享免费代测实验服务，可检测多种属样本。全程提供。如需其他elisa试剂盒说明书及报价，可我公司!
安吉到龙门货运专线回程车_安吉到惠州龙门物流公司
油泵P2105R00D1C30LB20V00C1B1U现货库存
合肥到福清专线物流公司_合肥到福清物流专线[直达运输]
济南到东莞石龙镇整车物流货运专线_济南到石龙物流公司
高密到横州货运专线展会运输_高密到横州物流公司
小鼠胰岛素（INS）酶联免疫试剂盒-齐一生物
成都到昌吉物流专线直达_优质成都到昌吉物流公司
上海金山区到马山县物流专线直达_优质上海金山区到南宁马山县物流公司
宣城到广州海珠区物流专线直达_优质宣城到海珠区物流公司
上海宝山区到当阳物流专线直达_专业上海宝山区到当阳物流公司
巢湖到宽城货运专线返程配货_巢湖到承德宽城物流公司
江门到重庆大足区货运专线_江门到大足区物流公司「专线直达」
珠海到西安雁塔区物流公司专线运输,珠海到雁塔区物流专线
晋江到农安县物流公司,优质晋江到吉林农安县物流专线公司
厦门到巢湖货运回头车_从厦门发货巢湖的物流公司
太原到安泽县专线物流运输_太原到临汾安泽县物流专线公司
徐州到天津南开区物流专线#在线报价_徐州到南开区物流直达专线
嘉善到阜城物流专线#在线报价_嘉善到衡水阜城物流直达专线
苏州到上海宝山区货运专线_优质苏州到宝山区物流专线公司
嘉善到石狮货运专线展会运输_嘉善到石狮物流公司
仅供研究用
货号：qy-m30038
小鼠胰岛素(ins)酶联免疫分析(elisa)
试剂盒使用说明书
本试剂仅供研究使用 目的：本试剂盒用于测定小鼠血清，细胞上清及相关液体样本中胰岛素(ins)的含量。
实验原理：
本试剂盒应用双抗体夹心法测定标本中小鼠胰岛素(ins)水平。用纯化的小鼠抗-胰岛素(ins)抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入胰岛素(ins),再与hrp 标记的胰岛素（ins）抗体结合，形成抗体-抗原-酶标抗体复合物，经过*洗涤后加底物 tmb 显色。tmb 在hrp 酶的催化下转化成蓝色，并在酸的作用下转化成zui终的黄色。颜色的深浅和样品中的胰岛素(ins)呈正相关。用酶标仪在 450nm波长下测定吸光度（od值），通过标准曲线计算样品中小鼠胰岛素(ins)浓度。
specimen requirements
n
assay procedure
1.dilute and add sample to standard: set 10 standard wells on the elisa plates coated, add standard 100μl to the first and the second well, then add standard dilution 50μl to the first and the second well, mix; take out 100μl form the first and the second well then add it to the third and the forth well separay. then add standard dilution 50μl to the third and the forth well ,mix ; then take out 50μl from the third and the forth well discard, add 50μl to the fifth and the sixth well ,then add standard dilution 50μl to the fifth and the sixth well, mix ; take out 50μl from the fifth and the sixth well and add to the seventh and the eighth well, then add standard dilution 50μl to the seventh and the eighth well ,mix ; take out 50μl from the seventh and the eighth well and add to the ninth and the tenth well, add standard dilution 50μl to the ninth and the tenth well, mix , take out 50μl from the ninth and the tenth well discard(add sample 50μl to each well after diluting ,(density: 12 mu/l,8mu/l ,4 mu/l,2 mu/l, 1 mu/l)
2.add sample ：set blank wells separay (blank comparison wells don’t add sample and hrp-conjugate reagent, other each step operation is same). testing sample well. add sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, and gently mix.
3.add enzyme：add hrp-conjugate reagent 50μl to each well, except blank well.
4.incubate: after closing plate with closure plate membrane ,incubate for 30 min at 37℃. 5.configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water and reserve.
6.washing：uncover closure plate membrane, discard liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.
7.color：add chromogen solution a 50ul and chromogen solution b to each well, evade thelight preservation for 10 min at 37℃
8.stop the reaction ：add stop solution50μl to each well, stop the reaction(the blue color change to yellow color).
9.assay：take blank well as zero , read absorbance at 450nm after adding stop solution and within 15min.
important notes
the kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, elisa plates coated if has not use up after opened, the plate should be stored in sealed bag.washing buffer will crystallization separation, it can be heated the water helps dissolve when dilute . washing does not affect the result.add sample with sampler each step, and proofread its accuracy frequently, avoids the experimental error. add sample within 5 min, if the number of sample is much , recommend to use volley .if the testing material content is excessively higher (the sample od is bigger than the first standard well ),please dilute sample (n-fold), please diluente and multiplied by the dilution factor.（×n×5）.closure plate membrane only limits the disposable use, to avoid cross-contamination.the substrate evade the light preservation.please according to use instruction strictly, the test result determination must take the microtiter plate reader as a standard.all samples, washing buffer and each kind of reject should according to infective material process.do not mix reagents with those from other lots.assay range
0.3 mu/l -15 mu/l
storage and validity
1．storage： 2-8℃.
2．validity：six months.
齐一生物科技（上海）有限公司主要经营进口原装elisa试剂盒，自产品牌qiybo试剂盒，并提供免疫分析相关试剂和检测试剂盒。种类齐全，价格实惠，凡购买我公司产品的新老客户，即可享免费代测实验服务，可检测多种属样本。全程提供。如需其他elisa试剂盒说明书及报价，可我公司!
安吉到龙门货运专线回程车_安吉到惠州龙门物流公司
油泵P2105R00D1C30LB20V00C1B1U现货库存
合肥到福清专线物流公司_合肥到福清物流专线[直达运输]
济南到东莞石龙镇整车物流货运专线_济南到石龙物流公司
高密到横州货运专线展会运输_高密到横州物流公司
小鼠胰岛素（INS）酶联免疫试剂盒-齐一生物
成都到昌吉物流专线直达_优质成都到昌吉物流公司
上海金山区到马山县物流专线直达_优质上海金山区到南宁马山县物流公司
宣城到广州海珠区物流专线直达_优质宣城到海珠区物流公司
上海宝山区到当阳物流专线直达_专业上海宝山区到当阳物流公司
巢湖到宽城货运专线返程配货_巢湖到承德宽城物流公司
江门到重庆大足区货运专线_江门到大足区物流公司「专线直达」
珠海到西安雁塔区物流公司专线运输,珠海到雁塔区物流专线
晋江到农安县物流公司,优质晋江到吉林农安县物流专线公司
厦门到巢湖货运回头车_从厦门发货巢湖的物流公司
太原到安泽县专线物流运输_太原到临汾安泽县物流专线公司
徐州到天津南开区物流专线#在线报价_徐州到南开区物流直达专线
嘉善到阜城物流专线#在线报价_嘉善到衡水阜城物流直达专线
苏州到上海宝山区货运专线_优质苏州到宝山区物流专线公司
嘉善到石狮货运专线展会运输_嘉善到石狮物流公司