期刊:protoplasma
主题:高温和干旱胁迫导致水稻脱落酸和活性氧的积累,抑制种子萌发生长
标题:high temperature and drought stress cause abscisic acid and reactive oxygen species accumulation and suppress seed germination growth in rice.
影响因子:2.633
检测指标:ca2+、h2o2流速
检测部位:水稻胚芽鞘出苗位点
ca2+、h2o2流速流实验处理方法:
种子分别在高温38摄氏度,20%peg-6000处理5天
ca2+、h2o2流速流实验测试液成份:
0.1 mm cacl2, 0.1 mm kcl,0.3mm mes, ph 6.0
作者:河南农业大学赵全志、刘娟
英文摘要
seed germination is one of the most important biological processes in the life cycle of plants, and temperature and water are the two most critical environmental factors that influence seed germination.
in the present study, we investigated the roles of the plant hormone abscisic acid (aba) and reactive oxygen species (ros) in high temperature (ht) and drought-induced inhibition of rice seed germination. ht and drought stress caused aba accumulation in seeds and inhibited seed germination and seedling establishment. quantitative real-time polymerase chain reaction analysis revealed that ht and drought stress induced the expression of osnced3, a key gene in aba synthesis in rice seeds.
in addition, ros (o2•- and h2o2) and malondialdehyde contents were increased in germinating seeds under ht and drought stress. moreover, we adopted the non-invasive micro-test technique to detect h2o2 and ca2+ fluxes at the site of coleoptile emergence. ht and drought stress resulted in a h2o2 efflux, but only drought stress significantly induced ca2+ influx. antioxidant enzyme assays revealed that superoxide dismutase (sod), peroxidase, catalase (cat), and ascorbate peroxidase (apx) activity were reduced by ht and drought stress, consistent with the expression of oscu/znsod, oscatc, and osapx2 during seed germination.
altogether, these results suggest that aba and ros accumulation under ht and drought conditions can inhibit rice seed germination and growth.
中文摘要
种子萌发是植物生命周期中重要的生物过程之一,温度和水是影响种子萌发的两个关键的环境因素。
在本研究中,我们研究了植物激素脱落酸(aba)和活性氧(ros)在高温(ht)和干旱诱导的水稻种子萌发抑制中的作用。 ht和干旱胁迫导致种子中aba积累,抑制种子萌发和幼苗建立。定量实时聚合酶链反应分析表明,ht和干旱胁迫诱导水稻种子aba合成中关键基因osnced3的表达。
此外,在ht和干旱胁迫下,萌发种子中ros(o2• - 和h2o2)和丙二醛含量增加。此外,我们采用非侵入性微测试技术检测胚芽鞘出现部位的h2o2和ca2+通量。 ht和干旱胁迫导致h2o2流出,但只有干旱胁迫才能显着诱导ca2 +流入。抗氧化酶测定表明,ht和干旱胁迫使超氧化物歧化酶(sod),过氧化物酶,过氧化氢酶(cat)和抗坏血酸过氧化物酶(apx)活性降低,与种子萌发过程中oscu / znsod,oscatc和osapx2的表达一致。
总之,这些结果表明,在ht和干旱条件下aba和ros的积累可以抑制水稻种子的萌发和生长。
结果表明:干旱胁迫显着加剧了发芽种子中胚芽鞘出苗位点的ca2+净吸收(图b),ca2+净吸收量比对照种子高61.2%。同时,ht对ca2+净流入没有显着影响,尽管与对照相比略有下降。 h2o2流速在对照组和治疗组之间的方向和大小上显示出显着差异(图c)。对照种子表现出h2o2的吸收。然而,在ht和干旱胁迫下,h2o2从种子外排到测试溶液中,分别达到1.20和0.44pmol cm-2 s -1。这些结果可能表明h2o2外排进一步意味着在ht和干旱胁迫下发芽种子中积累更多的h2o含量。
地埋箱泵一体化水池技术研究
PC DBCO-PEG3-biotin的介绍
蝶阀安装维护
恒温恒湿试验箱的原理及结构
使用盐雾腐蚀试验箱的注意事项
河南农大丨高温干旱胁迫致ABA和ROS积累影响水稻萌发
双金属温度计WSS-302参数
常见应力分析方法归纳
内蒙古碳达峰方案出炉:2030年城市绿色出行比例达到70%
KRACHT流量计配套底板的尺寸以及选型
电子材料试验机综述
国产压力传感器与进口压力传感器综合比较
恒温振荡培养箱的日常使用操作应注意些什么呢
人防丙种射线监测仪是监测什么的及作用是哪些?
分析不同型号回路电阻测试仪测出示值的差异现象
人脸识别测温一体机-人脸识别测温面板机
驻马店AT8900呼气式酒精检测仪更换电池厂家
冷却水塔外防腐 烟筒安装旋转梯
环氧乙烯基玻璃鳞片涂料耐酸涂料
胜伟厂家生产自锁压板,低碳钢保温钩钉