ScreenFect A：高效且低毒的脂质体，转染间充质干细胞
screenfect a: an efficient and low toxic liposome for gene delivery to mesenchymal stem cells
screenfect a：一种高效且低毒的脂质体，用于将基因传递到间充质干细胞
摘要
间充质干细胞（mscs）在包括组织工程和癌症治疗在内的各种治疗应用中具有巨大的前景。间充质干细胞的基因修饰可用于通过促进特定功能或将间充质干细胞转化为更有效的基因治疗工具来增强间充质干细胞的治疗效果。然而，转基因msc的成功生成往往受到现有转染试剂转染效率差或毒性高的限制。在我们之前的研究中，我们使用硫醇-炔点击化学来开发新的脂质体载体，包括screenfect（a） （® sf）（li等人，2012）。本研究考察了sf对mscs的转染性能。以lipofectamine™ 2000（l2k）为对照，对转染效率、细胞活力和细胞dna摄取进行了比较评估，结果表明sf在msc转染方面优于l2k。血清的存在对sf或l2k的转染效率没有显著影响，但大大降低了l2k转染msc的活力。与l2k相比，sf介导的转染效率更高，也与更好的细胞增殖相关。这些结果得到了dna细胞内命运监测的支持，这证实了dna从溶酶体的稳定运输和有效的核定位。sf的tgf-β1基因递送促进了成骨诱导条件下msc成骨分化。作为sf脂质化对干细胞的研究，本研究强调了sf在向间充质干细胞和其他干细胞的基因传递中促进基于转基因干细胞的组织工程和其他治疗效果的有希望的作用。
abstract
mesenchymal stem cells (mscs) hold great promise in variety of therapeutic applications including tissue engineering and cancer therapy. genetic modification of mscs can be used to enhance the therapeutic effect of mscs by facilitating a specific function or by transforming mscs into more effective gene therapy tools. however, the successful generation of genetically modified mscs is often limited by the poor transfection efficiency or high toxicity of available transfection reagents. in our previous study, we used thiol-yne click chemistry to develop new liposomal vectors, including screenfect(®) a (sf) (li et al., 2012). in this study, we investigated the transfection performance of sf on mscs. a comparative evaluation of transfection efficiency, cell viability and cellular dna uptake was performed using the lipofectamine™ 2000 (l2k) as a control, and the results show that sf is superior to l2k for msc transfection. the presence of serum did not significantly influence the transfection efficiency of either sf or l2k but greatly reduced the viability of msc transfected by l2k. the higher efficiency of sf-mediated transfection compared to l2k was also correlated with better proliferation of cells. these results were supported by monitoring the intracellular fate of dna, which confirmed stable transportation of dna from lysosomes and efficient nuclear localization. tgf-β1 gene delivery by sf promoted msc osteogenic differentiation in an osteogenic induction condition. as the first study of sf lipofection on stem cells, this study highlights a promising role of sf in gene delivery to mscs as well as other stem cells to facilitate tissue engineering and other therapeutic effects based on genetically modified stem cells.
通过科研成果转化，screenfect已经成为一种商业转染试剂。德国screentfect联合靶点科技，将这种通过点击化学筛选出来的高效转染试剂带给中国广大的科研工作者。详情联系靶点科技。
大型冲床对于拖链选择需要做好哪些
电厂输煤转运站干雾抑尘原理
进口电动移液器具有多功能移液模式
半导体材料切削液-水分质量分数的测定
电子秤（电子台秤）的正确充电和保养方法
ScreenFect A：高效且低毒的脂质体，转染间充质干细胞
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常见的这几类花生类食品，在真空包装机选择上有哪些区别？
大气采样仪的使用保养
DANFOSS丹佛斯卸压阀的工作原理及操作方法
有机废气治理技术
德国SAMSON萨姆森3730-3电动气动定位器工作原理
真空捏合机机件润滑说明
提供：超细无机纤维喷涂棉价格-报价表
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pj新申请承装修试四级需要哪些条件
蜜二糖Melibiose用作特殊培养基的主要应用
screenfect a：一种高效且低毒的脂质体，用于将基因传递到间充质干细胞
摘要
间充质干细胞（mscs）在包括组织工程和癌症治疗在内的各种治疗应用中具有巨大的前景。间充质干细胞的基因修饰可用于通过促进特定功能或将间充质干细胞转化为更有效的基因治疗工具来增强间充质干细胞的治疗效果。然而，转基因msc的成功生成往往受到现有转染试剂转染效率差或毒性高的限制。在我们之前的研究中，我们使用硫醇-炔点击化学来开发新的脂质体载体，包括screenfect（a） （® sf）（li等人，2012）。本研究考察了sf对mscs的转染性能。以lipofectamine™ 2000（l2k）为对照，对转染效率、细胞活力和细胞dna摄取进行了比较评估，结果表明sf在msc转染方面优于l2k。血清的存在对sf或l2k的转染效率没有显著影响，但大大降低了l2k转染msc的活力。与l2k相比，sf介导的转染效率更高，也与更好的细胞增殖相关。这些结果得到了dna细胞内命运监测的支持，这证实了dna从溶酶体的稳定运输和有效的核定位。sf的tgf-β1基因递送促进了成骨诱导条件下msc成骨分化。作为sf脂质化对干细胞的研究，本研究强调了sf在向间充质干细胞和其他干细胞的基因传递中促进基于转基因干细胞的组织工程和其他治疗效果的有希望的作用。
abstract
mesenchymal stem cells (mscs) hold great promise in variety of therapeutic applications including tissue engineering and cancer therapy. genetic modification of mscs can be used to enhance the therapeutic effect of mscs by facilitating a specific function or by transforming mscs into more effective gene therapy tools. however, the successful generation of genetically modified mscs is often limited by the poor transfection efficiency or high toxicity of available transfection reagents. in our previous study, we used thiol-yne click chemistry to develop new liposomal vectors, including screenfect(®) a (sf) (li et al., 2012). in this study, we investigated the transfection performance of sf on mscs. a comparative evaluation of transfection efficiency, cell viability and cellular dna uptake was performed using the lipofectamine™ 2000 (l2k) as a control, and the results show that sf is superior to l2k for msc transfection. the presence of serum did not significantly influence the transfection efficiency of either sf or l2k but greatly reduced the viability of msc transfected by l2k. the higher efficiency of sf-mediated transfection compared to l2k was also correlated with better proliferation of cells. these results were supported by monitoring the intracellular fate of dna, which confirmed stable transportation of dna from lysosomes and efficient nuclear localization. tgf-β1 gene delivery by sf promoted msc osteogenic differentiation in an osteogenic induction condition. as the first study of sf lipofection on stem cells, this study highlights a promising role of sf in gene delivery to mscs as well as other stem cells to facilitate tissue engineering and other therapeutic effects based on genetically modified stem cells.
通过科研成果转化，screenfect已经成为一种商业转染试剂。德国screentfect联合靶点科技，将这种通过点击化学筛选出来的高效转染试剂带给中国广大的科研工作者。详情联系靶点科技。
大型冲床对于拖链选择需要做好哪些
电厂输煤转运站干雾抑尘原理
进口电动移液器具有多功能移液模式
半导体材料切削液-水分质量分数的测定
电子秤（电子台秤）的正确充电和保养方法
ScreenFect A：高效且低毒的脂质体，转染间充质干细胞
贴铝箔橡塑保温板厂家生产厂家地址
常见的这几类花生类食品，在真空包装机选择上有哪些区别？
大气采样仪的使用保养
DANFOSS丹佛斯卸压阀的工作原理及操作方法
有机废气治理技术
德国SAMSON萨姆森3730-3电动气动定位器工作原理
真空捏合机机件润滑说明
提供：超细无机纤维喷涂棉价格-报价表
新乡玻璃鳞片防腐胶泥阻燃胶泥
恒温恒湿箱不制冷怎么办？
ASCO淹没式脉冲阀安装的几种方式
圆锥球磨机应该如何操作？
pj新申请承装修试四级需要哪些条件
蜜二糖Melibiose用作特殊培养基的主要应用