如何高效率转染人结直肠癌细胞
西南大学生命科学学院*创新重点实验室
使用zeta life，advanceddnarna转染试剂
高效率转染hct-116, sw480, ht-29人结直肠癌细胞
2020-4-28发表文章已见刊
发表文章转染条件
a、hct-116, sw480, ht-29人结直肠癌细胞
b、aurka质粒
c、转染细胞融合度50%
d、96孔板每孔使用0.5μg质粒dna
6孔每孔使用10 ug质粒dna
(注意：本实验中用到的细胞密度、转染质粒dna用量不适用于lipo3000/2000)
发表文章部分内容
overexpression of aurkaplasmid for aurka overexpression was obtained fromvectorbuilder. details about plasmid containing the aurka gene canbe found at colon cancer cells (hct-116, sw480, ht-29)were seeded at 50% confluency for 24 h in six-well and 96-well plates. plasmid (10 ug per-well for six-well and 0.5μg per-well for96 plates) and theadvanceddnarnatransfection（zeta life,usa) reagent were mixed in equal proportionsand added to the cell culture medium for 6 h. dox (1μg/ml) was addedto induce the expression of aurka. after addition of dox and pal for36 h, cell viability was measured by mtt and apoptosis was detected by。
pal exerts anti-colon cancer effects by targeting aurka. (a) pal promotes apoptosis in colon cancer cells (hct-116, sw480) in a dose-dependent manner.(b) pal promotes g2/m phase arrest in colon cancer cells (hct-116, sw480) in a dose-dependent manner. (c) the expression of aurka after dox treatment for24 h. (d) cell density after pal treatment for 24 h in the cells (hct-116, sw480) transfected with aurka overexpression plasmids. dox is an inducer of aurka
expression in plasmids. (e) effect of pal detected by mtt assay on the proliferation ability of colon cancer cells transfected with aurka overexpression plasmids. (f)effects of pal detected by.
zeta life，advanced dna rna转染试剂信息如下：
产品名称
货号
规格
advanced dna rna 转染试剂
ad600025
0.25ml
advanced dna rna 转染试剂
ad600050
0.5
advanced dna rna 转染试剂
ad600075
0.75
advanced dna rna 转染试剂
ad600150
1.5ml
advanced dna rna 转染试剂
ad600500
5ml
advanced dna rna 转染试剂
ad601000
10ml
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点火电磁阀-VGP15R02W6仓库充满现货
如何高效率转染人结直肠癌细胞
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船用法兰介绍
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尿素吸料机
GB5009.35-2023食品中合成着色剂整体解决方案
使用zeta life，advanceddnarna转染试剂
高效率转染hct-116, sw480, ht-29人结直肠癌细胞
2020-4-28发表文章已见刊
发表文章转染条件
a、hct-116, sw480, ht-29人结直肠癌细胞
b、aurka质粒
c、转染细胞融合度50%
d、96孔板每孔使用0.5μg质粒dna
6孔每孔使用10 ug质粒dna
(注意：本实验中用到的细胞密度、转染质粒dna用量不适用于lipo3000/2000)
发表文章部分内容
overexpression of aurkaplasmid for aurka overexpression was obtained fromvectorbuilder. details about plasmid containing the aurka gene canbe found at colon cancer cells (hct-116, sw480, ht-29)were seeded at 50% confluency for 24 h in six-well and 96-well plates. plasmid (10 ug per-well for six-well and 0.5μg per-well for96 plates) and theadvanceddnarnatransfection（zeta life,usa) reagent were mixed in equal proportionsand added to the cell culture medium for 6 h. dox (1μg/ml) was addedto induce the expression of aurka. after addition of dox and pal for36 h, cell viability was measured by mtt and apoptosis was detected by。
pal exerts anti-colon cancer effects by targeting aurka. (a) pal promotes apoptosis in colon cancer cells (hct-116, sw480) in a dose-dependent manner.(b) pal promotes g2/m phase arrest in colon cancer cells (hct-116, sw480) in a dose-dependent manner. (c) the expression of aurka after dox treatment for24 h. (d) cell density after pal treatment for 24 h in the cells (hct-116, sw480) transfected with aurka overexpression plasmids. dox is an inducer of aurka
expression in plasmids. (e) effect of pal detected by mtt assay on the proliferation ability of colon cancer cells transfected with aurka overexpression plasmids. (f)effects of pal detected by.
zeta life，advanced dna rna转染试剂信息如下：
产品名称
货号
规格
advanced dna rna 转染试剂
ad600025
0.25ml
advanced dna rna 转染试剂
ad600050
0.5
advanced dna rna 转染试剂
ad600075
0.75
advanced dna rna 转染试剂
ad600150
1.5ml
advanced dna rna 转染试剂
ad600500
5ml
advanced dna rna 转染试剂
ad601000
10ml
动态消毒水工作站延长香梨饮料食品保质期，杀菌消毒
SMC双联气缸技术参数
大型潜水轴流泵出现故障怎么办？
油料光谱仪的油液检测典型应用
点火电磁阀-VGP15R02W6仓库充满现货
如何高效率转染人结直肠癌细胞
冷拔无缝钢管力学性能
如何安装E+H涡街流量计
沥青混合料离心抽提仪
船用法兰介绍
HUBNER编码器HOG71DN1000TTL笑容写在心里
巴鲁夫BALLUFF超声波传感器
如何合理配置采石场的石料生产线？
简单讲述荧光定量 PCR 原理
数控磨床 外圆磨床在砂轮上如何修整
上医治未病 治之于未燃 | 电子探针精准锁定锂电池起火潜藏诱因
“5G＋医疗”商用潜力待掘，走向成熟需三管齐下
高智能食品安全检测仪-高智能食品安全检测仪-2020新品*
尿素吸料机
GB5009.35-2023食品中合成着色剂整体解决方案