Nucleosome, Recombinant Human, H2BK120ub1 dNuc, Biotinylated
epicypher是一家为表观遗传学和染色质生物学研究提供高质量试剂和工具的专业制造商。epicypher生产的在e. coli中表达的重组人单核小体(组蛋白h2a、h2b、h3和h4各2个;accession numbers:h2a-p04908;h2b-o60814;h3.1-p68431;h4-p62805)由601位处的147个dna碱基对缠绕在组蛋白上构成。组蛋白h2b(由专门的半合成方法产生)在120位处含有泛素赖氨酸。核小体是染色质的基本单位，lowary和widom鉴定的601序列是一个含有147碱基对的序列，对组蛋白八聚体具有高亲和力，可用于核小体组装，并含有5'biotin-teg基团。适合用作酶筛选分析或效应蛋白结合实验的底物。
[1] lowary and widom j. mol. biol. (1998). pmid: 9514715
产品详情
标签: biotinylated
保存温度:stable for six months at -80°c from date of receipt. for best results, aliquot and avoid multiple freeze/thaws.
产品形式:h2bk120ub dnuc (14.4 µg protein weight, 25 µg dna + protein) in 22.5 µl 10 mm tris hcl ph 7.5, 25 mm nacl, 1 mm edta, 2 mm dtt, 20% glycerol. molarity = 5.12 μm. mw = 216,985.9 da.
验证数据
figure 1: western blot data
western analysis of nucleosome, recombinant human, h2bk120ub.
top panel: unmodified h3 (lane 1) and h2bk120ub containing nucleosomes
(lane 2) were probed with an anti-h2bk120ub antibody and analyzed via ecl
readout. only the h2bk120ub sample produced a detectable signal. bottom
panel: detail from coomassie stained gel showing unmodified nucleosomes
(lane 1) and h2bk120ub nucleosomes (lane 2).
figure 2: mass spec datah2bk120ub protein analyzed by high
resolution mass spectrometry. expected mass = 22,321.8 da. determined
mass = 22,320.3 da.
figure 3: protein gel data
coomassie stained page gel of proteins in nucleosome, recombinant
human, h2bk120ub (1 μg) to demonstrate the purity of the histones in the
preparation. sizes of molecular weight markers and positions of the core
histones (h2a, h2bk120ub, h3.1, and h4) are indicated.
figure 4: dna gel data
nucleosome, recombinant human, h2bk120ub dnucs resolved via native
page and stained with ethidium bromide to visualize dna. lane 1: free
dna (100 ng). lane 2: intact h2bk120ub nucleosome (400 ng).
订购详情
货号
产品名称
规格
16-0396
nucleosome, recombinant human, h2bk120ub1 dnuc, biotinylated
25µg
如需了解更多详细信息或相关产品，请联系epicypher中国授权代理商-欣博盛生物
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Nucleosome, Recombinant Human, H2BK120ub1 dNuc, Biotinylated
10t/d一体化生活污水处理设备工艺
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[1] lowary and widom j. mol. biol. (1998). pmid: 9514715
产品详情
标签: biotinylated
保存温度:stable for six months at -80°c from date of receipt. for best results, aliquot and avoid multiple freeze/thaws.
产品形式:h2bk120ub dnuc (14.4 µg protein weight, 25 µg dna + protein) in 22.5 µl 10 mm tris hcl ph 7.5, 25 mm nacl, 1 mm edta, 2 mm dtt, 20% glycerol. molarity = 5.12 μm. mw = 216,985.9 da.
验证数据
figure 1: western blot data
western analysis of nucleosome, recombinant human, h2bk120ub.
top panel: unmodified h3 (lane 1) and h2bk120ub containing nucleosomes
(lane 2) were probed with an anti-h2bk120ub antibody and analyzed via ecl
readout. only the h2bk120ub sample produced a detectable signal. bottom
panel: detail from coomassie stained gel showing unmodified nucleosomes
(lane 1) and h2bk120ub nucleosomes (lane 2).
figure 2: mass spec datah2bk120ub protein analyzed by high
resolution mass spectrometry. expected mass = 22,321.8 da. determined
mass = 22,320.3 da.
figure 3: protein gel data
coomassie stained page gel of proteins in nucleosome, recombinant
human, h2bk120ub (1 μg) to demonstrate the purity of the histones in the
preparation. sizes of molecular weight markers and positions of the core
histones (h2a, h2bk120ub, h3.1, and h4) are indicated.
figure 4: dna gel data
nucleosome, recombinant human, h2bk120ub dnucs resolved via native
page and stained with ethidium bromide to visualize dna. lane 1: free
dna (100 ng). lane 2: intact h2bk120ub nucleosome (400 ng).
订购详情
货号
产品名称
规格
16-0396
nucleosome, recombinant human, h2bk120ub1 dnuc, biotinylated
25µg
如需了解更多详细信息或相关产品，请联系epicypher中国授权代理商-欣博盛生物
持粘性测定仪依据GB23441、GB/T4851标准粘合力测试
脱硫曝气风机在脱硫氧化中的作用用途及工作原理
LT-Z235 呼吸管路综合性测试仪 YY0461-2003 产品介绍
CPC直线导轨最大作用
德国KUBLER库伯勒编码器的常见故障7个点来描述
Nucleosome, Recombinant Human, H2BK120ub1 dNuc, Biotinylated
10t/d一体化生活污水处理设备工艺
聚氨酯直埋保温管保温特性
聚氨酯保温管的生产加快了保温工程的施工速度
分享一些非离子聚丙烯酰胺相关知识，希望对大家有所帮助
如何选择实用的电池片拉力试验机
测磷仪 煤中磷测定仪
联轴器的举例说明
内园切片机的发展和现状
SF6微水测试仪测量原理
人造肉入选2018新兴科技 配备哪些设备可入局
移动式龙门架
太原礼堂椅厂家
血红蛋白分析仪的基本原理
AcuPM473-U，AcuPM473-U三相电压传感器