艾美捷萤火虫荧光素酶测定试剂盒中英文介绍
firefly luciferase is widely used as a reporter gene to study gene regulation and function and drug screening. since there is no endogenous luciferase activity in mammalian cells or tissues, it is a very sensitive genetic reporter gene. firefly luciferase is a 62000 dalton protein, which is active as a monomer, and does not require subsequent processing of its activity. this enzyme catalyzes the oxidation of atp dependent d-luciferin to oxidized luciferin, producing light emission. the firefly luciferase follows the michaelis menten kinetics, so the maximum light output can be achieved only when the substrate and cofactor are in large excess. when analyzing under these conditions, the light emitted by the reaction is proportional to the number of luciferase molecules.
note: this is a flash type luminescence detection that requires the signal to be measured immediately after adding the working solution to the sample. it requires a single tube luminescent meter or luminescent enzyme reader with a reagent syringe. biotium also offers the steady luc hts firefly detection kit (product number 30028l), which is a homogeneous luminescent detection with a signal half-life of 3-5 hours.
easy to use for consistent results
the firefly luciferase detection kit is designed to simply and effectively quantify the activity of firefly luciferase reporter enzyme in cultured cells, with high sensitivity and linearity. this kit is a new improved substitute for the firefly luciferase test kit (catalog no. 30003). it adopts firefly assay buffer 2.0, which has better working solution stability compared to the original detection buffer.
other commercial luciferase tests require you to dilute all firefly substrates in the test buffer, resulting in a limited shelf life after first use. in biotium's reagent kit, the substrate is provided as a separate stock solution, which is freshly diluted for each test to achieve excellent shelf life and provide consistent signals over time.
the measurement size is based on the 96 hole format. this kit includes sufficient cell lysis buffer to perform the specified number of measurements within the size range of 96 well to 24 well plates.
萤火虫荧光素酶测定试剂盒2.0是用于转染细胞裂解物中萤火虫荧光素酶活性的闪光型测定。该试剂盒是我们最初的萤火虫荧光素酶测定（目录号30003）的改进版本，具有更好的工作溶液稳定性。150次测定。
艾美捷萤火虫荧光素酶测定试剂盒2.0,150次测定#btm-30085-1组件：
5x 萤火虫萤光素酶裂解缓冲液
萤火虫萤光素酶检测缓冲液2.0
d-荧光素
萤火虫荧光素酶测定试剂盒相关特征：
高灵敏度和线性闪光型测定
每次检测都添加新鲜的底物，以确保信号随时间推移保持一致
高性价比
该萤火虫荧光素酶测定试剂盒属性：
化验类型/选项：终点测定，发光（闪光型）
检测方法/读数：光度计（带试剂注射器的单管或微量板读取器）
储存条件：在-60°c或更低温度下储存
多参数水质测定仪器
介绍FLC系列防爆滤油机车的产品参数
应用案例|步进频率连续波雷达在非金属管道和地下分层的应用
铝合金基材 密封胶铝基材的使用说明
费斯托FESTO电磁阀的产品特点与那些？
艾美捷萤火虫荧光素酶测定试剂盒中英文介绍
微射流高压均质机在虾青素纳米脂质体中的应用
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生活饮用水无毒防腐管道诚源厂家阳光明媚
热收缩带的施工流程
外墙真金板直销
【解决方案】吹田电气 | EV、HEV 电池的放电测量
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note: this is a flash type luminescence detection that requires the signal to be measured immediately after adding the working solution to the sample. it requires a single tube luminescent meter or luminescent enzyme reader with a reagent syringe. biotium also offers the steady luc hts firefly detection kit (product number 30028l), which is a homogeneous luminescent detection with a signal half-life of 3-5 hours.
easy to use for consistent results
the firefly luciferase detection kit is designed to simply and effectively quantify the activity of firefly luciferase reporter enzyme in cultured cells, with high sensitivity and linearity. this kit is a new improved substitute for the firefly luciferase test kit (catalog no. 30003). it adopts firefly assay buffer 2.0, which has better working solution stability compared to the original detection buffer.
other commercial luciferase tests require you to dilute all firefly substrates in the test buffer, resulting in a limited shelf life after first use. in biotium's reagent kit, the substrate is provided as a separate stock solution, which is freshly diluted for each test to achieve excellent shelf life and provide consistent signals over time.
the measurement size is based on the 96 hole format. this kit includes sufficient cell lysis buffer to perform the specified number of measurements within the size range of 96 well to 24 well plates.
萤火虫荧光素酶测定试剂盒2.0是用于转染细胞裂解物中萤火虫荧光素酶活性的闪光型测定。该试剂盒是我们最初的萤火虫荧光素酶测定（目录号30003）的改进版本，具有更好的工作溶液稳定性。150次测定。
艾美捷萤火虫荧光素酶测定试剂盒2.0,150次测定#btm-30085-1组件：
5x 萤火虫萤光素酶裂解缓冲液
萤火虫萤光素酶检测缓冲液2.0
d-荧光素
萤火虫荧光素酶测定试剂盒相关特征：
高灵敏度和线性闪光型测定
每次检测都添加新鲜的底物，以确保信号随时间推移保持一致
高性价比
该萤火虫荧光素酶测定试剂盒属性：
化验类型/选项：终点测定，发光（闪光型）
检测方法/读数：光度计（带试剂注射器的单管或微量板读取器）
储存条件：在-60°c或更低温度下储存
多参数水质测定仪器
介绍FLC系列防爆滤油机车的产品参数
应用案例|步进频率连续波雷达在非金属管道和地下分层的应用
铝合金基材 密封胶铝基材的使用说明
费斯托FESTO电磁阀的产品特点与那些？
艾美捷萤火虫荧光素酶测定试剂盒中英文介绍
微射流高压均质机在虾青素纳米脂质体中的应用
维谛NetSure211 C23智能化通信电源 维谛48v40A嵌入式电源的使用方法@技术快讯
选择双锥干燥机需要检验哪些事项呢？
智能光照培养箱是具有生化功能的高精度恒温设备
制造医疗器械用不锈钢针管韧性测试仪 介绍
HYDAC贺德克反冲洗过滤器安装技巧及方法
SH-01纺织品原料回潮率测定仪怎么测试Z准确
阐述智能金属管浮子流量计使用中的注意事项
生活饮用水无毒防腐管道诚源厂家阳光明媚
热收缩带的施工流程
外墙真金板直销
【解决方案】吹田电气 | EV、HEV 电池的放电测量
不锈钢混动气旋塔工作原理
沥青运动粘度计使用方法