anti-pro-sp-c - rabbit, n-terminal (wrab-9337)使用说明
产品信息
品牌
seven hills bioreagents
货号
wrab-9337
规格
100 µl
宿主
兔
反应种属
人、鼠
克隆类型
多克隆
偶 联 物
无
缓冲物
0.01% sodium azide
抗原
重组鼠肺表面活性蛋白c前体pro-sp-c, n-terminal (aa1-35) gst融合蛋白
保存条件
储存在-20℃,避免反复冻融
产品使用
实验应用
建议稀释倍数
western blot
1:5000
immunohistochemistry
1:3000
说明:最佳稀释倍数与浓度应由研究人员确认
应用实例
图片为成年小鼠肺组织用抗n-末端prosp-c兔血清(1:3000稀释)染色。prosp-c通过dab(黑色)进行可视化,并用nuclear fast red(粉红色)进行复染。放大倍数40倍。
参考文献
1. glasser sw, detmer ea, ikegami m, na cl, stahlman mt, whitsett ja. 'pneumonitis and emphysema in sp-c gene targeted mice.' j biol chem. 2003 apr 18;278(16):14291-8.
2. nogee lm, dunbar ae 3rd, wert se, askin f, hamvas a, whitsett ja. 'a mutation in the surfactant protein c gene associated with familial interstitial lung disease.' n engl j med. 2001 feb 22;344(8):573-9.
3. no abstract available. glasser sw, burhans ms, korfhagen tr, na cl, sly pd, ross gf, ikegami m, whitsett ja. 'altered stability of pulmonary surfactant in sp-c-deficient mice.' proc natl acad sci u s a. 2001 may 22;98(11):6366-71.
4. zhou l, lim l, costa rh, whitsett ja. 'thyroid transcription factor-1, hepatocyte nuclear factor-3beta, surfactant protein b, c, and clara cell secretory protein in developing mouse lung.' j histochem cytochem. 1996 oct;44(10):1183-93.
5. yang, l., j. johansson, r. ridsdale, h. willander, m. fitzen, h. t. akinbi, and t. e. weaver. surfactant protein b propeptide contains a saposin-like protein domain with antimicrobial activity at low ph. j immunol 2010;184:975-983.
6. conkright, j. j., k. s. apsley, e. p. martin, r. ridsdale, w. r. rice, c. l. na, b. yang, and t. e. weaver. nedd4-2-mediated ubiquitination facilitates processing of surfactant protein-c. am j respir cell mol biol 2010;42:181-189.
7. wang, m., j. p. bridges, c. l. na, y. xu, and t. e. weaver. meckel-gruber syndrome protein mks3 is required for endoplasmic reticulum-associated degradation of surfactant protein c. j biol chem 2009;284:33377-33383.
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