EpiCypher&欣博盛生物 DNA纯化试剂盒介绍

采用专为低洗脱体积(低至6µl)设计的锥形净化柱,可回收>50bp的dna片段,只需30分钟即可完成纯化流程,是各种cut&run靶点,包括组蛋白ptm和转录因子的理想选择。
图例:
figure 1:cut&run dna fragment size distribution analysis
500,000 k562 cells per sample were processed with the cutana™ chic/cut&run kit (epicypher 14-1048) following treatment with igg negative control (epicypher 13-0042), h3k4me3 (epicypher 13-0041), h3k27me3 (epicypher 13-0030) and ctcf (epicypher 13-2014) antibodies. isolated dna was used to prepare paired-end illumina® sequencing libraries, which were analyzed by agilent tapestation® , confirming enrichment of mononucleosomes (~300 bp peak represents ~170 bp excised dna + sequencing adapters).
产品描述:
cleavage under targets & release using nuclease (cut&run) utilizes an immunotethering approach to specifically release genomic fragments of interest into solution, enabling next-generation sequencing and genomic mapping with unprecedented sensitivity. high yield purification and concentration of partitioned chromatin dna is an essential step - one that requires a workflow specifically compatible with low starting material. utilizing tapered cleanup columns designed for low elution volumes (as low as 6 µl), this kit produces purified, concentrated dna ready for library preparation and next-generation sequencing (figure 1). the user-friendly workflow can be completed in just 30 minutes. the columns recover > 50 bp dna fragments, making the dna purification kit ideal for a variety of cut&run targets, including histone ptms and transcription factors.
产品订购
产品名称 货号 规格
cutana™ dna purification kit 14-0050 50 preps
如需了解更多详细信息或相关产品,请联系epicypher中国授权代理商-欣博盛生物

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