Human Motilin（MTL）试剂盒
human motilin（mtl）
for research use only
assay range：20 pg/ml -480 pg/ml 96 determinations
purpose
this kit allows for the determination of mtl concentrations in human serum, cell culture supernates and other biological fluids
principle of the assay
the kit assay human motilin（mtl） level in the sample，use purified human motilin（mtl）antibody to coat microtiter plate wells, make solid-phase antibody, then add human motilin（mtl） to wells, combined antibody which with hrp labeled goat anti- human become antibody - antigen - enzyme-antibody complex, after washing compley, add tmb substrate solution,tmb substrate becomes blue color at hrp enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. the concentration of human motilin（mtl） in the samples is then determined by comparing the o.d. of the samples to the standard curve.
materials provided with the kit
1
wash solution
20ml×1bottle
7
stopp solution
6ml×1 bottle
2
hrp-conjugate reagent
6ml×1 bottle
8
standard（960pg/ml）
0.5ml×1 bottle
3
microelisa stripplate
12well×8strips
9
standard diluent
1.5ml×1bottle
4
sample diluent
6ml×1 bottle
10
instruction
1
5
chromogen solution a
6ml×1 bottle
11
closure plate membrane
2
6
chromogen solution b
6ml×1 bottle
12
sealed bags
1
specimen requirements
extract as soon as possible after specimen collection,and according to the relevant literature, and should be experiment as soon as possible after the extraction. if it can’t, specimen can be kept in -20 ℃ to preserve, avoid repeated freeze-thaw cycles.can’t detect the sample which contain nan3, because nan3 inhibits hrp active.assay procedure
dilute and add sample:dilute original density standard as follow table:480pg/ml
5 standard
150μl original density standard+150μl standard diluent
240 pg/ml
4 standard
150μl 5 standard+150μl standard diluent
120 pg/ml
3 standard
150μl 4 standard+150μl standard diluent
60 pg/ml
2 standard
150μl 3 standard +150μl standard diluent
30 pg/ml
1 standard
150μl 2 standard +150μl standard diluent
2.add sample：set blank wells separay （blank comparison wells don’t add sample and hrp-conjugate reagent, other each step operation is same）. testing sample well. add sample dilution 40μl to testing sample well, then add testing sample 10μl （sample final dilution is 5-fold）, add sample to wells , don’t touch the well wall as far as possible, and gently mix.
3.incubate: after closing plate with closure plate membrane ,incubate for 30 min at 37℃.
4.configurate liquid: 30-fold wash solution diluted 30-fold （or 20-fold） with distilled water and reserve.
5.washing：uncover closure plate membrane, discard liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.
6.add enzyme：add hrp-conjugate reagent 50μl to each well, except blank well.
7.incubate：operation with 3.
8.washing：operation with 5.
9.color：add chromogen solution a 50ul and chromogen solution b to each well, evade the light preservation for 15 min at 37℃
10.stop the reaction：add stop solution50μl to each well, stop the reaction（the blue color change to yellow color）.
11.assay：take blank well as zero , read absorbance at 450nm after adding stop solution and within 15min.
steps description
standard, sample diluent
add standard, sample diluent, incubate for 30 min at 37℃.
wash 5 time,add hrp-conjugate reagent, incubate for 30 min at 37℃.
wash 5 times,add chromogen solution a and b, incubate for 30 min at 37℃.
add stopp solution
read absorbance at 450nm within 15 min
calculate
calculate
take the standard density as the horizontal, the od value for the vertical ,draw the standard curve on graph paper, find out the corresponding density according to the sample od value by the sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density and the od value ,with the sample od value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.
important notes
the kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, elisa plates coated if has not use up after opened, the plate should be stored in sealed bag.washing buffer will crystallization separation, it can be heated the water helps dissolve when dilute . washing does not affect the result.add sample with sampler each step, and proofread its accuracy frequently, avoids the experimental error. add sample within 5 min, if the number of sample is much , recommend to use volley .if the testing material content is excessively higher （the sample od is bigger than the first standard well ）,please dilute sample （n-fold）, please diluente and multiplied by the dilution factor.（×n×5）.closure plate membrane only limits the disposable use, to avoid cross-contamination.the substrate evade the light preservation.please according to use instruction strictly, the test result determination must take the microtiter plate reader as a standard.all samples, washing buffer and each kind of reject should according to infective material process.do not mix reagents with those from other lots.
storage and validity
1．storage： 2-8℃.
2．validity： six months
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for research use only
assay range：20 pg/ml -480 pg/ml 96 determinations
purpose
this kit allows for the determination of mtl concentrations in human serum, cell culture supernates and other biological fluids
principle of the assay
the kit assay human motilin（mtl） level in the sample，use purified human motilin（mtl）antibody to coat microtiter plate wells, make solid-phase antibody, then add human motilin（mtl） to wells, combined antibody which with hrp labeled goat anti- human become antibody - antigen - enzyme-antibody complex, after washing compley, add tmb substrate solution,tmb substrate becomes blue color at hrp enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. the concentration of human motilin（mtl） in the samples is then determined by comparing the o.d. of the samples to the standard curve.
materials provided with the kit
1
wash solution
20ml×1bottle
7
stopp solution
6ml×1 bottle
2
hrp-conjugate reagent
6ml×1 bottle
8
standard（960pg/ml）
0.5ml×1 bottle
3
microelisa stripplate
12well×8strips
9
standard diluent
1.5ml×1bottle
4
sample diluent
6ml×1 bottle
10
instruction
1
5
chromogen solution a
6ml×1 bottle
11
closure plate membrane
2
6
chromogen solution b
6ml×1 bottle
12
sealed bags
1
specimen requirements
extract as soon as possible after specimen collection,and according to the relevant literature, and should be experiment as soon as possible after the extraction. if it can’t, specimen can be kept in -20 ℃ to preserve, avoid repeated freeze-thaw cycles.can’t detect the sample which contain nan3, because nan3 inhibits hrp active.assay procedure
dilute and add sample:dilute original density standard as follow table:480pg/ml
5 standard
150μl original density standard+150μl standard diluent
240 pg/ml
4 standard
150μl 5 standard+150μl standard diluent
120 pg/ml
3 standard
150μl 4 standard+150μl standard diluent
60 pg/ml
2 standard
150μl 3 standard +150μl standard diluent
30 pg/ml
1 standard
150μl 2 standard +150μl standard diluent
2.add sample：set blank wells separay （blank comparison wells don’t add sample and hrp-conjugate reagent, other each step operation is same）. testing sample well. add sample dilution 40μl to testing sample well, then add testing sample 10μl （sample final dilution is 5-fold）, add sample to wells , don’t touch the well wall as far as possible, and gently mix.
3.incubate: after closing plate with closure plate membrane ,incubate for 30 min at 37℃.
4.configurate liquid: 30-fold wash solution diluted 30-fold （or 20-fold） with distilled water and reserve.
5.washing：uncover closure plate membrane, discard liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.
6.add enzyme：add hrp-conjugate reagent 50μl to each well, except blank well.
7.incubate：operation with 3.
8.washing：operation with 5.
9.color：add chromogen solution a 50ul and chromogen solution b to each well, evade the light preservation for 15 min at 37℃
10.stop the reaction：add stop solution50μl to each well, stop the reaction（the blue color change to yellow color）.
11.assay：take blank well as zero , read absorbance at 450nm after adding stop solution and within 15min.
steps description
standard, sample diluent
add standard, sample diluent, incubate for 30 min at 37℃.
wash 5 time,add hrp-conjugate reagent, incubate for 30 min at 37℃.
wash 5 times,add chromogen solution a and b, incubate for 30 min at 37℃.
add stopp solution
read absorbance at 450nm within 15 min
calculate
calculate
take the standard density as the horizontal, the od value for the vertical ,draw the standard curve on graph paper, find out the corresponding density according to the sample od value by the sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density and the od value ,with the sample od value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.
important notes
the kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, elisa plates coated if has not use up after opened, the plate should be stored in sealed bag.washing buffer will crystallization separation, it can be heated the water helps dissolve when dilute . washing does not affect the result.add sample with sampler each step, and proofread its accuracy frequently, avoids the experimental error. add sample within 5 min, if the number of sample is much , recommend to use volley .if the testing material content is excessively higher （the sample od is bigger than the first standard well ）,please dilute sample （n-fold）, please diluente and multiplied by the dilution factor.（×n×5）.closure plate membrane only limits the disposable use, to avoid cross-contamination.the substrate evade the light preservation.please according to use instruction strictly, the test result determination must take the microtiter plate reader as a standard.all samples, washing buffer and each kind of reject should according to infective material process.do not mix reagents with those from other lots.
storage and validity
1．storage： 2-8℃.
2．validity： six months
检查调节阀的步骤
使用地埋一体化污水处理设备时需要注意哪些要点？
HJW-60（30）强制式单卧轴混凝土搅拌机
焊接机器人的结构是怎样的?
盛夏时分智能家居有多热？烽火点燃
Human Motilin（MTL）试剂盒
对激光切割机切割速度的理解一起来看看
非洲猪瘟pcr检测仪便携式好用吗？
施耐德电气终端配电家族全新亮相 两大产品全新升级
冷冻后细胞会复苏吗
ATOS比例阀AGMZO-AE-10/315 10使用注意事项
机器视觉系统的主要工作过程原理
点胶设备智能自动化发展
智能闸机IC卡和ID卡介绍区别？
德国REXROTH功率放大器如何驱动超声波换能器
医药冷库的电控部分
如何把不锈钢蝶阀的销售和互联网联络在一起
楼梯滑动支座聚四氟乙烯板报价
HPC001S新拌混凝土测试仪
LN2低温液氮减压阀的特征